DNA was isolated using AMPure XP beads as described for the input sample. For each nChIP\seq, 0.5?l of each sample was used for qPCR validation of enrichment at control regions. of both marks during XCI, albeit with distinct genomic distributions. Furthermore, using a B Acemetacin (Emflex) and C repeat mutant, which still shows gene silencing … Continue reading DNA was isolated using AMPure XP beads as described for the input sample